Evidence for a link between gut microbiota and hypertension in the Dahl rat.

mrc2admin's picture
Tue, 2015-09-15 14:22 -- mrc2admin
TitleEvidence for a link between gut microbiota and hypertension in the Dahl rat.
Publication TypeJournal Article
Year of Publication2015
AuthorsMell B, Jala VR, Mathew AV, Byun J, Waghulde H, Zhang Y, Haribabu B, Vijay-Kumar M, Pennathur S, Joe B
JournalPhysiol Genomics
Volume47
Issue6
Pagination187-97
Date Published2015 Jun
ISSN1531-2267
Abstract

The gut microbiota plays a critical role in maintaining physiological homeostasis. This study was designed to evaluate whether gut microbial composition affects hypertension. 16S rRNA genes obtained from cecal samples of Dahl salt-sensitive (S) and Dahl salt-resistant (R) rats were sequenced. Bacteria of the phylum Bacteroidetes were higher in the S rats compared with the R rats. Furthermore, the family S24-7 of the phylum Bacteroidetes and the family Veillonellaceae of the phylum Firmicutes were higher in the S rats compared with the R rats. Analyses of the various phylogenetic groups of cecal microbiota revealed significant differences between S and R rats. Both strains were maintained on a high-salt diet, administered antibiotics for ablation of microbiota, transplanted with S or R rat cecal contents, and monitored for blood pressure (BP). Systolic BP of the R rats remained unaltered irrespective of S or R rat cecal transplantation. Surprisingly, compared with the S rats given S rat cecal content, systolic BP of the S rats given a single bolus of cecal content from R rats was consistently and significantly elevated during the rest of their life, and they had a shorter lifespan. A lower level of fecal bacteria of the family Veillonellaceae and increased plasma acetate and heptanoate were features associated with the increased BP observed in the S rats given R rat microbiota compared with the S rats given S rat microbiota. These data demonstrate a link between microbial content and BP regulation and, because the S and R rats differ in their genomic composition, provide the necessary basis to further examine the relationship between the host genome and microbiome in the context of BP regulation in the Dahl rats.

DOI10.1152/physiolgenomics.00136.2014
Alternate JournalPhysiol. Genomics
PubMed ID25829393
PubMed Central IDPMC4451389
Grant ListDK-08194 / DK / NIDDK NIH HHS / United States
DK-097153 / DK / NIDDK NIH HHS / United States
DK-89503 / DK / NIDDK NIH HHS / United States
HL-020176 / HL / NHLBI NIH HHS / United States
HL-076709 / HL / NHLBI NIH HHS / United States
HL-112641 / HL / NHLBI NIH HHS / United States