RhoC is a Potent Regulator of Glutamine Metabolism and N-acetylaspartate Production in Inflammatory Breast Cancer Cells.

Fri, 2016-06-24 11:53 -- voskuhlt
TitleRhoC is a Potent Regulator of Glutamine Metabolism and N-acetylaspartate Production in Inflammatory Breast Cancer Cells.
Publication TypeJournal Article
Year of Publication2016
AuthorsWynn ML, Yates JA, Evans CR, Van Wassenhove L, Wu ZFen, Bridges S, Bao L, Fournier C, Ashrafzadeh S, Merrins MJ, Satin LS, Schnell S, Burant CF, Merajver SD
JournalJ Biol Chem
Date Published2016 Apr 25
ISSN1083-351X
Abstract

Inflammatory breast cancer (IBC) is an extremely lethal cancer that rapidly metastasizes. While the molecular attributes of IBC have been described, little is known about the underlying metabolic features of the disease. Using a variety of metabolic assays including (13)C tracer experiments, we found that SUM149 cells, the primary in vitro model of IBC, exhibit metabolic abnormalities that distinguish them from other breast cancer cells, including elevated levels of N-acetylaspartate (NAA), a metabolite primarily associated with neuronal disorders and gliomas. Here we provide the first evidence of NAA in breast cancer. We also report that the oncogene RhoC, a driver of metastatic potential, modulates glutamine and N-acetylaspartate metabolism in IBC cells in vitro, revealing a novel role for RhoC as a regulator of tumor cell metabolism that extends beyond its well-known role in cytoskeletal rearrangement.

DOI10.1074/jbc.M115.703959
Alternate JournalJ. Biol. Chem.
PubMed ID27129239